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Cytotoxic Effect of Drosophila Deoxynucleoside Kinase Gene on Replicating Plasmid in HeLa Cells
Title: | Cytotoxic Effect of Drosophila Deoxynucleoside Kinase Gene on Replicating Plasmid in HeLa Cells |
Authors: | Ito, Mana Browse this author | Suda, Yusuke Browse this author | Harashima, Hideyoshi Browse this author | Kamiya, Hiroyuki Browse this author |
Keywords: | replicating plasmid | deoxynucleoside kinase | cell death |
Issue Date: | 1-Jul-2010 |
Journal Title: | Biological & Pharmaceutical Bulletin |
Volume: | 33 |
Issue: | 7 |
Start Page: | 1223 |
End Page: | 1227 |
Publisher DOI: | 10.1248/bpb.33.1223 |
Abstract: | To enhance the levels of transgene expression from plasmid-based nonviral vectors, replicating plasmids containing the SV40 origin and the SV40 large T antigen gene, as a model replicating unit, were constructed. The replicating luciferase plasmid DNA produced the luciferase protein more efficiently than the non-replicating luciferase plasmid DNA, as expected. Surprisingly, the introduction of the replicating plasmid DNA containing the Drosophila melanogaster deoxyribonucleoside kinase (Dm-dNK) gene was highly cytotoxic and caused cell death without nucleoside analogs. Our results confirm that transgenes on a replicating plasmid represent an excellent tool for effective protein production and suggest that efficient production of the Dm-dNK protein in tumor cells could be an attractive cancer therapy. |
Type: | article |
URI: | http://hdl.handle.net/2115/43201 |
Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 紙谷 浩之
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