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Cytotoxic Effect of Drosophila Deoxynucleoside Kinase Gene on Replicating Plasmid in HeLa Cells

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Title: Cytotoxic Effect of Drosophila Deoxynucleoside Kinase Gene on Replicating Plasmid in HeLa Cells
Authors: Ito, Mana Browse this author
Suda, Yusuke Browse this author
Harashima, Hideyoshi Browse this author
Kamiya, Hiroyuki Browse this author
Keywords: replicating plasmid
deoxynucleoside kinase
cell death
Issue Date: 1-Jul-2010
Journal Title: Biological & Pharmaceutical Bulletin
Volume: 33
Issue: 7
Start Page: 1223
End Page: 1227
Publisher DOI: 10.1248/bpb.33.1223
Abstract: To enhance the levels of transgene expression from plasmid-based nonviral vectors, replicating plasmids containing the SV40 origin and the SV40 large T antigen gene, as a model replicating unit, were constructed. The replicating luciferase plasmid DNA produced the luciferase protein more efficiently than the non-replicating luciferase plasmid DNA, as expected. Surprisingly, the introduction of the replicating plasmid DNA containing the Drosophila melanogaster deoxyribonucleoside kinase (Dm-dNK) gene was highly cytotoxic and caused cell death without nucleoside analogs. Our results confirm that transgenes on a replicating plasmid represent an excellent tool for effective protein production and suggest that efficient production of the Dm-dNK protein in tumor cells could be an attractive cancer therapy.
Type: article
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷 浩之

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