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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >
反射出血を利用した昆虫類のDNA解析
| Title: | 反射出血を利用した昆虫類のDNA解析 |
| Authors: | 加藤, 徹 Browse this author | | 古川, 恒太 Browse this author | | 片倉, 晴雄 Browse this author →KAKEN DB |
| Keywords: | reflex bleeding | | DNA extraction | | PCR | | DNA sequencing |
| Issue Date: | 25-Mar-2008 |
| Publisher: | 日本昆虫学会 |
| Journal Title: | 昆蟲. ニューシリーズ |
| Volume: | 11 |
| Issue: | 1 |
| Start Page: | 25 |
| End Page: | 31 |
| Abstract: | Reflex bleeding is characteristic for a number of insect species such as ladybeetles and leaf beetles. In this study, we assessed the utility of reflex bleeding as a noninvasive source for DNA analysis. By using the method based on the nucleotide-acid binding property of silica particles, we were able to extract DNA from the reflex bleeding exuded by some ladybeetles. In addition, using the extracted DNA, we were able to amplify and determine the nucleotide sequences of 28S region. These results suggest that the reflex bleeding will be a useful source for analyzing DNA in insects without killing them. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/43827 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 加藤 徹
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