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Sperm dimorphism in terms of nuclear shape and microtubule accumulation in Cyrtanthus mackenii

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Title: Sperm dimorphism in terms of nuclear shape and microtubule accumulation in Cyrtanthus mackenii
Authors: Hirano, Tomonari Browse this author
Hoshino, Yoichiro Browse this author →KAKEN DB
Keywords: Cyrtanthus mackenii
male germ unit
pollen tube
sperm dimorphism
Issue Date: Mar-2010
Publisher: Springer
Journal Title: Sexual Plant Reproduction
Volume: 23
Issue: 2
Start Page: 153
End Page: 162
Publisher DOI: 10.1007/s00497-009-0123-2
PMID: 20490968
Abstract: Pollen tubes of Cyrtanthus mackenii, a species with bicellular pollen, were cultured in vitro to investigate nuclear phase changes during generative cell division and male germ unit (MGU) formation using flow cytometric analysis. Results revealed that sperm cells were formed after 12 h of culture. During sperm maturation, the nuclei of sperm cells were not associated with the vegetative nucleus (unassociated sperm cells; Sua) and became longer than those of sperm cells associated with the vegetative nucleus (Svn). These findings indicate that the pair of sperm cells in the C. mackenii MGU is dimorphic in terms of nuclear shape. Dimorphism coincides with anti-α-tubulin antibody immunofluorescence, which was higher in the Sua than in Svn. Following treatment with oryzalin, triggering microtubule depolymerization, differences between nuclear shape in the two sperm nuclei disappeared, suggesting that microtubule accumulation between sperm cells in the MGU correlates with differences in the nuclear shape.
Rights: The original publication is available at
Type: article (author version)
Appears in Collections:北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 星野 洋一郎

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