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Enhanced transgene expression from chromatinized plasmid DNA in mouse liver

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Title: Enhanced transgene expression from chromatinized plasmid DNA in mouse liver
Authors: Kamiya, Hiroyuki Browse this author →KAKEN DB
Miyamoto, Shiho Browse this author
Goto, Hitomi Browse this author
Kanda, Genki N. Browse this author
Kobayashi, Miwako Browse this author
Matsuoka, Ichiro Browse this author
Harashima, Hideyoshi Browse this author →KAKEN DB
Keywords: Exogenous DNA
Histone
Nucleosome
Intranuclear disposition
Expression efficiency
Issue Date: 30-Jan-2013
Publisher: Elsevier B.V.
Journal Title: International Journal of Pharmaceutics
Volume: 441
Issue: 1-2
Start Page: 146
End Page: 150
Publisher DOI: 10.1016/j.ijpharm.2012.12.004
Abstract: Plasmid DNA was chromatinized with core histones (H2A, H2B, H3, and H4) in vitro and was delivered into mouse liver by hydrodynamics-based administration. Transgene expression from the chromatinized plasmid DNA was more efficient than that from plasmid DNA delivered in the naked form. The use of acetylation-enriched histones isolated from cells treated with a histone deacetylase inhibitor (trichostatin A) seemed to be more effective. These results indicated that chromatinized plasmid DNA is useful for efficient transgene expression in vivo.
Type: article (author version)
URI: http://hdl.handle.net/2115/52137
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷 浩之

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