Hokkaido University Collection of Scholarly and Academic Papers >
Faculty of Pharmaceutical Sciences >
Peer-reviewed Journal Articles, etc >
Enhanced transgene expression from chromatinized plasmid DNA in mouse liver
Title: | Enhanced transgene expression from chromatinized plasmid DNA in mouse liver |
Authors: | Kamiya, Hiroyuki Browse this author →KAKEN DB | Miyamoto, Shiho Browse this author | Goto, Hitomi Browse this author | Kanda, Genki N. Browse this author | Kobayashi, Miwako Browse this author | Matsuoka, Ichiro Browse this author | Harashima, Hideyoshi Browse this author →KAKEN DB |
Keywords: | Exogenous DNA | Histone | Nucleosome | Intranuclear disposition | Expression efficiency |
Issue Date: | 30-Jan-2013 |
Publisher: | Elsevier B.V. |
Journal Title: | International Journal of Pharmaceutics |
Volume: | 441 |
Issue: | 1-2 |
Start Page: | 146 |
End Page: | 150 |
Publisher DOI: | 10.1016/j.ijpharm.2012.12.004 |
Abstract: | Plasmid DNA was chromatinized with core histones (H2A, H2B, H3, and H4) in vitro and was delivered into mouse liver by hydrodynamics-based administration. Transgene expression from the chromatinized plasmid DNA was more efficient than that from plasmid DNA delivered in the naked form. The use of acetylation-enriched histones isolated from cells treated with a histone deacetylase inhibitor (trichostatin A) seemed to be more effective. These results indicated that chromatinized plasmid DNA is useful for efficient transgene expression in vivo. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/52137 |
Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 紙谷 浩之
|