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A cis-acting element in the coding region of cyclin B1 mRNA couples subcellular localization to translational timing
| Title: | A cis-acting element in the coding region of cyclin B1 mRNA couples subcellular localization to translational timing |
| Authors: | Yasuda, Kyota Browse this author | | Kotani, Tomoya Browse this author →KAKEN DB | | Yamashita, Masakane Browse this author →KAKEN DB |
| Keywords: | RNA localization | | Cyclin B1 | | Oocyte maturation | | Translational control |
| Issue Date: | 15-Oct-2013 |
| Publisher: | Academic press inc elsevier science |
| Journal Title: | Developmental biology |
| Volume: | 382 |
| Issue: | 2 |
| Start Page: | 517 |
| End Page: | 529 |
| Publisher DOI: | 10.1016/j.ydbio.2013.05.014 |
| PMID: | 23701882 |
| Abstract: | Subcellular localization of messenger RNAs (mRNAs) to correct sites and translational activation at appropriate timings are crucial for normal progression of various biological events. However, a molecular link between the spatial regulation and temporal regulation remains unresolved. In immature zebrafish oocytes, translationally repressed cyclin B1 mRNA is localized to the animal polar cytoplasm and its temporally regulated translational activation in response to a maturationAnducing hormone is essential to promote oocyte maturation. We previously reported that the coding region of cyclin B1 mRNA is required for the spatio-temporal regulation. Here, we report that a sequence, CAGGAGACC, that is conserved in the coding region of vertebrate cyclin B1 mRNA is involved in the regulation. Like endogenous cyclin B1 mRNA, reporter mRNAs harboring the sequence CAGGAGACC were localized to the animal polar cytoplasm of oocytes, while those carrying mutations in the sequence (with no change in the coding amino acids) were dispersed in the animal hemisphere of oocytes. Furthermore, translational activation of the mutant mRNAs was initiated at a timing earlier than that of endogenous and wild-type reporter mRNAs during oocyte maturation. Interaction of CAGGAGACC with proteins in vitro suggests that this sequence functions in collaboration with a trans-acting protein factor(s) in oocytes. These findings reveal that the sequence in the coding region of cyclin B1 mRNA plays an important role as a cis-acting element in both subcellular localization and translational timing of mRNA, providing a direct molecular link between the spatial and temporal regulation of mRNA translation. (C) 2013 Elsevier Inc. All rights reserved. |
| Relation: | http://www.elsevier.com/wps/find/authorsview.authors/rights | | http://www.sciencedirect.com/science/article/pii/S0012160613002595 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/53625 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山下 正兼
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