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c-Myc gene single-strand binding protein-1, MSSP-1, suppresses transcription of alpha-smooth muscle actin gene in chicken visceral smooth muscle cells.
| Title: | c-Myc gene single-strand binding protein-1, MSSP-1, suppresses transcription of alpha-smooth muscle actin gene in chicken visceral smooth muscle cells. |
| Authors: | Kimura, Kazuhiro Browse this author | | Saga, Hiroshi Browse this author | | Hayashi, Ken'ichiro Browse this author →KAKEN DB | | Obata, Hideto Browse this author | | Chimori, Yoshihiro Browse this author | | Ariga, Hiroyoshi Browse this author →KAKEN DB | | Sobue, Kenji Browse this author →KAKEN DB |
| Issue Date: | 15-May-1998 |
| Publisher: | Oxford University Press |
| Journal Title: | Nucleic acids research |
| Volume: | 26 |
| Issue: | 10 |
| Start Page: | 2420 |
| End Page: | 2425 |
| Publisher DOI: | 10.1093/nar/26.10.2420 |
| PMID: | 9580695 |
| Abstract: | The expression of alpha-smooth muscle actin is coordinately regulated by positive and negative cis- elements in the promoter region. Although cis -elements and trans -acting factors involved in the positive regulation of the alpha-smooth muscle (alpha-SM) actin gene have been well characterized, details of negative regulation remain unclear. In functional analyses using cultured gizzard smooth muscle cells, we identified a sequence ranging from -238 to -219 in the promoter region as a novel negative element. Mutation and deletion analyses further revealed that a sequence, TATCTTA (-228 to -222), is essential for negative regulation. Gel shift assay and Southwestern blotting indicated that a nuclear protein factor specifically interacts with single- or double-strand DNA including this sequence, and the protein factor displays a highly potent binding to the sense strand DNA. cDNA cloning and gel shift analysis using anti-MSSP-1 antibodies revealed that this protein factor is a chicken homolog of human MSSP-1 (c- myc gene single-strand binding protein-1). In fact, overexpression of MSSP-1 in cultured smooth muscle cells suppresses the promoter activity. These results suggest a novel function of MSSP-1 regarding the transcriptional regulation of alpha-sm actin gene. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/53971 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 有賀 寛芳
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