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c-Myc gene single-strand binding protein-1, MSSP-1, suppresses transcription of alpha-smooth muscle actin gene in chicken visceral smooth muscle cells.

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Title: c-Myc gene single-strand binding protein-1, MSSP-1, suppresses transcription of alpha-smooth muscle actin gene in chicken visceral smooth muscle cells.
Authors: Kimura, Kazuhiro Browse this author
Saga, Hiroshi Browse this author
Hayashi, Ken'ichiro Browse this author →KAKEN DB
Obata, Hideto Browse this author
Chimori, Yoshihiro Browse this author
Ariga, Hiroyoshi Browse this author →KAKEN DB
Sobue, Kenji Browse this author →KAKEN DB
Issue Date: 15-May-1998
Publisher: Oxford University Press
Journal Title: Nucleic acids research
Volume: 26
Issue: 10
Start Page: 2420
End Page: 2425
Publisher DOI: 10.1093/nar/26.10.2420
PMID: 09580695
Abstract: The expression of alpha-smooth muscle actin is coordinately regulated by positive and negative cis- elements in the promoter region. Although cis -elements and trans -acting factors involved in the positive regulation of the alpha-smooth muscle (alpha-SM) actin gene have been well characterized, details of negative regulation remain unclear. In functional analyses using cultured gizzard smooth muscle cells, we identified a sequence ranging from -238 to -219 in the promoter region as a novel negative element. Mutation and deletion analyses further revealed that a sequence, TATCTTA (-228 to -222), is essential for negative regulation. Gel shift assay and Southwestern blotting indicated that a nuclear protein factor specifically interacts with single- or double-strand DNA including this sequence, and the protein factor displays a highly potent binding to the sense strand DNA. cDNA cloning and gel shift analysis using anti-MSSP-1 antibodies revealed that this protein factor is a chicken homolog of human MSSP-1 (c- myc gene single-strand binding protein-1). In fact, overexpression of MSSP-1 in cultured smooth muscle cells suppresses the promoter activity. These results suggest a novel function of MSSP-1 regarding the transcriptional regulation of alpha-sm actin gene.
Type: article
URI: http://hdl.handle.net/2115/53971
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 有賀 寛芳

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