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3,3 ',5,5 '-Tetramethylbenzidine Oxidation on Paper Devices for Horseradish Peroxidase-based Assays

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Title: 3,3 ',5,5 '-Tetramethylbenzidine Oxidation on Paper Devices for Horseradish Peroxidase-based Assays
Authors: Busa, Lori Shayne Alamo Browse this author
Komatsu, Takeshi Browse this author
Mohammadi, Saeed Browse this author
Maeki, Masatoshi Browse this author
Ishida, Akihiko Browse this author
Tani, Hirofumi Browse this author
Tokeshi, Manabu Browse this author →KAKEN DB
Keywords: TMB oxidation
horseradish peroxidase
paper-based analytical devices
photolithography
wax-printing
Issue Date: Aug-2016
Publisher: 日本分析化学会
Journal Title: Analytical sciences
Volume: 32
Issue: 8
Start Page: 815
End Page: 818
Publisher DOI: 10.2116/analsci.32.815
PMID: 27506705
Abstract: We report on the colorimetric oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by hydrogen peroxide using horseradish peroxidase on photolithography-fabricated (P-PAD) and wax-printed (W-PAD) paper-based analytical devices. Fabricating PADs via photolithography exposes the hydrophilic areas to polymers (photoresists) and solvents, not only reducing the hydrophilicity, but also affecting the TMB-H2O2 assay system with an unavoidable incomplete elimination of photoresist during fabrication. Detection signals are then observed in the presence of photoresist residues on the P-PAD, even at a blank HRP concentration.
Type: article
URI: http://hdl.handle.net/2115/63206
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次 学

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