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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >
3,3 ',5,5 '-Tetramethylbenzidine Oxidation on Paper Devices for Horseradish Peroxidase-based Assays
| Title: | 3,3 ',5,5 '-Tetramethylbenzidine Oxidation on Paper Devices for Horseradish Peroxidase-based Assays |
| Authors: | Busa, Lori Shayne Alamo Browse this author | | Komatsu, Takeshi Browse this author | | Mohammadi, Saeed Browse this author | | Maeki, Masatoshi Browse this author | | Ishida, Akihiko Browse this author | | Tani, Hirofumi Browse this author | | Tokeshi, Manabu Browse this author →KAKEN DB |
| Keywords: | TMB oxidation | | horseradish peroxidase | | paper-based analytical devices | | photolithography | | wax-printing |
| Issue Date: | Aug-2016 |
| Publisher: | 日本分析化学会 |
| Journal Title: | Analytical sciences |
| Volume: | 32 |
| Issue: | 8 |
| Start Page: | 815 |
| End Page: | 818 |
| Publisher DOI: | 10.2116/analsci.32.815 |
| PMID: | 27506705 |
| Abstract: | We report on the colorimetric oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by hydrogen peroxide using horseradish peroxidase on photolithography-fabricated (P-PAD) and wax-printed (W-PAD) paper-based analytical devices. Fabricating PADs via photolithography exposes the hydrophilic areas to polymers (photoresists) and solvents, not only reducing the hydrophilicity, but also affecting the TMB-H2O2 assay system with an unavoidable incomplete elimination of photoresist during fabrication. Detection signals are then observed in the presence of photoresist residues on the P-PAD, even at a blank HRP concentration. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/63206 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 渡慶次 学
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