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High-Speed In Situ Observation System for Sonoporation of Cells With Size- and Position-Controlled Microbubbles

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タイトル: High-Speed In Situ Observation System for Sonoporation of Cells With Size- and Position-Controlled Microbubbles
著者: Kudo, Nobuki 著作を一覧する
キーワード: Biological effects and dosimetry
biophysical mechanisms
ultrasound contrast agents
発行日: 2017年 1月
出版者: IEEE (Institute of Electrical and Electronics Engineers)
誌名: IEEE transactions on ultrasonics ferroelectrics and frequency control
巻: 64
号: 1
開始ページ: 273
終了ページ: 280
出版社 DOI: 10.1109/TUFFC.2016.2606551
抄録: A high-speed in situ microscopic observation system developed for basic studies on mechanisms of sonoporation is introduced in this paper. The main part of the system is an inverted-type fluorescence microscope, and a high-speed camera of 20 MHz in a maximum framing rate was used to visualize the dynamics of cavitation bubbles that causes a sonoporation effect. Differential interference contrast and fluorescence techniques were used for sensitive visualization of cell changes during sonoporation. The system is also equipped with optical tweezers that can move a microbubble of several microns in size by using a donut-shaped light beam. In situ microscopic observation of sonoporation was carried out using a cell with a size-and position-controlled microbubble. The experimental results showed that the ability of cells to repair sonoporation-induced damage depends on their membrane tension, indicating the usefulness of the observation system as a basic tool for the investigation of sonoporation phenomena.
Rights: © 2016 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 工藤 信樹


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