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Altered balance of epidermis-related chemokines in epidermolysis bullosa

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Title: Altered balance of epidermis-related chemokines in epidermolysis bullosa
Authors: Ujiie, Inkin Browse this author
Fujita, Yasuyuki Browse this author
Nakayama, Chihiro Browse this author
Matsumura, Wakana Browse this author
Suzuki, Shotaro Browse this author
Shinkuma, Satoru Browse this author →KAKEN DB
Nomura, Toshifumi Browse this author
Abe, Riichiro Browse this author →KAKEN DB
Shimizu, Hiroshi Browse this author →KAKEN DB
Keywords: Epidermolysis bullosa
Issue Date: Apr-2017
Publisher: Elsevier
Journal Title: Journal of dermatological science
Volume: 86
Issue: 1
Start Page: 37
End Page: 45
Publisher DOI: 10.1016/j.jdermsci.2016.12.021
PMID: 28094098
Abstract: Background: Epidennolysis bullosa (EB) is a congenital, refractory skin disease and there are no fundamental treatments. Recently, allogenic cell therapies are beginning to be applied as potential treatments, that are based on the concept that the allogenic cells can migrate into the skin and reconstitute the skin components. Although the mechanisms of cell migration into skin are not fully understood, chemokines are regarded as key factors in recruiting bone marrow-derived cells. Objectives: Our study aims to elucidate the expression of chemokines in the EB patients. Methods: We determined the expression of wound-healing related chemokines in the sera, keratinocytes, and skin tissues of EB patients and compared them to those of healthy volunteers by enzyme-linked immunosorbent assays, quantitative reverse transcription PCR, and immunofluorescence staining. Results: The serum levels of CXCL12 and HMGB1 were found to be significantly elevated in the EB patients. Conversely, the serum levels of CCL21 were found to be lower in the EB patients than in healthy controls. In addition, the serum levels of CXCL12 tended to increase and the serum levels of CCL27 tended to decrease with an increase in the affected body surface areas. To detect the origin of the circulating chemokines, we performed immunofluorescence staining. CCL21, CCL27, HMGB1 and CXCL12 were stained more broadly in the EB patient tissues than those in the control tissues. Conclusions: These results suggest that fluctuations in chemokine levels may contribute in a coordinated way to the wound-healing process and lend clues toward efficient cell therapies for EB.
Rights: © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 氏家 韻欣

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