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Determination of Peroxidase Encapsulated in Liposomes Using Homogentisic Acid .GAMMA.-Lactone Chemiluminescence

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Title: Determination of Peroxidase Encapsulated in Liposomes Using Homogentisic Acid .GAMMA.-Lactone Chemiluminescence
Authors: KAMIDATE, Tamio Browse this author
KIKUCHI, Nobuhito Browse this author
ISHIDA, Akihiko Browse this author
TANI, Hirofumi Browse this author →KAKEN DB
Keywords: Homogentisic acid γ-lactone (HAL)
chemiluminescence (CL)
determination of horseradish peroxidase (HRP)
Issue Date: Jun-2005
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 21
Issue: 6
Start Page: 701
End Page: 704
Publisher DOI: 10.2116/analsci.21.701
Abstract: Homogentisic acid γ-lactone (HAL) chemiluminescence (CL) was applied to the determination of horseradish peroxidase (HRP) encapsulated in liposomes. HRP was detected after the lysis of HRP-trapped liposomes with Triton X-100. CL response rate, detection limit and linear range of calibration curve for HRP in HAL CL were compared with those in p-iodophenol (p-IP)-enhanced luminol CL. Maximal light emission in HAL CL appeared more rapidly compared to that in p-IP enhanced luminol CL, thus resulting in remarkable reduction of CL measurement time. The detection limit for HRP in HAL CL was the same as that in p-IP-enhanced luminol CL. The linear range of calibration curve for HRP in HAL CL was improved by a factor of 50 compared with that in p-IP-enhanced luminol CL. From these results, it was found that HAL CL were superior to p-IP-enhanced luminol CL for the determination of HRP encapsulated in liposomes.
Type: article
URI: http://hdl.handle.net/2115/71701
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 谷 博文

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