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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >
Determination of Peroxidase Encapsulated in Liposomes Using Homogentisic Acid .GAMMA.-Lactone Chemiluminescence
| Title: | Determination of Peroxidase Encapsulated in Liposomes Using Homogentisic Acid .GAMMA.-Lactone Chemiluminescence |
| Authors: | KAMIDATE, Tamio Browse this author | | KIKUCHI, Nobuhito Browse this author | | ISHIDA, Akihiko Browse this author | | TANI, Hirofumi Browse this author →KAKEN DB |
| Keywords: | Homogentisic acid γ-lactone (HAL) | | chemiluminescence (CL) | | determination of horseradish peroxidase (HRP) |
| Issue Date: | Jun-2005 |
| Publisher: | 日本分析化学会 |
| Journal Title: | Analytical Sciences |
| Volume: | 21 |
| Issue: | 6 |
| Start Page: | 701 |
| End Page: | 704 |
| Publisher DOI: | 10.2116/analsci.21.701 |
| Abstract: | Homogentisic acid γ-lactone (HAL) chemiluminescence (CL) was applied to the determination of horseradish peroxidase (HRP) encapsulated in liposomes. HRP was detected after the lysis of HRP-trapped liposomes with Triton X-100. CL response rate, detection limit and linear range of calibration curve for HRP in HAL CL were compared with those in p-iodophenol (p-IP)-enhanced luminol CL. Maximal light emission in HAL CL appeared more rapidly compared to that in p-IP enhanced luminol CL, thus resulting in remarkable reduction of CL measurement time. The detection limit for HRP in HAL CL was the same as that in p-IP-enhanced luminol CL. The linear range of calibration curve for HRP in HAL CL was improved by a factor of 50 compared with that in p-IP-enhanced luminol CL. From these results, it was found that HAL CL were superior to p-IP-enhanced luminol CL for the determination of HRP encapsulated in liposomes. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71701 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 谷 博文
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