| Title: | A compact fluorescence polarization analyzer with high-transmittance liquid crystal layer |
| Authors: | Wakao, Osamu Browse this author |
| Satou, Ken Browse this author |
| Nakamura, Ayano Browse this author |
| Sumiyoshi, Ken Browse this author |
| Shirokawa, Masanori Browse this author |
| Mizokuchi, Chikaaki Browse this author |
| Shiota, Kunihiro Browse this author |
| Maeki, Masatoshi Browse this author |
| Ishida, Akihiko Browse this author |
| Tani, Hirofumi Browse this author |
| Shigemura, Koji Browse this author |
| Hibara, Akihide Browse this author |
| Tokeshi, Manabu Browse this author →KAKEN DB |
| Issue Date: | 27-Feb-2018 |
| Publisher: | American Institute of Physics (AIP) |
| Journal Title: | Review of Scientific Instruments |
| Volume: | 89 |
| Issue: | 2 |
| Start Page: | 024103 |
| Publisher DOI: | 10.1063/1.5017081 |
| PMID: | 29495833 |
| Abstract: | Fluorescence polarization (FP) offers easy operation and rapid processing, making it implementable in molecular interaction analysis. Previously we have developed a unique FP measurement system using a liquid crystal (LC) layer and an image sensor. The system is based on a principle of synchronized detection between the switching rate of the LC layer and the sampling rate of the CCD. The FP system realized simultaneous multiple sample detection; however, the measurement precision was lower than that of the conventional FP apparatus. The main drawbacks were low light transmittance of the LC layer and insufficient synchronization between the LC layer and CCD. In this paper, we developed a new FP analyzer based on LC-CCD synchronization detection. By using a newly designed LC with high transmittance and improving synchronization, the performance of the system has been dramatically improved. Additionally, we reduced the cost by using an inexpensive CCD and an LED as the excitation source. Simultaneous FP immunoassay of multiple samples of prostaglandin E2 was performed. The error rate of the FP system is reduced from 16.9% to 3.9%, as comparable to the commercial conventional FP system. Published by AIP Publishing. |
| Rights: | This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. The following article appeared in Review of Scientific Instruments 89, 024103 (2018) and may be found at https://doi.org/10.1063/1.5017081. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/72720 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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