Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >
Calcium-binding and structural stability of echidna and canine milk lysozymes
Title: | Calcium-binding and structural stability of echidna and canine milk lysozymes |
Authors: | Kikuchi, M. Browse this author | Kawano, K. Browse this author →KAKEN DB | Nitta, K.3 Browse this author →KAKEN DB |
Authors(alt): | 新田, 勝利3 |
Issue Date: | Oct-1998 |
Publisher: | Cambridge University Press |
Journal Title: | Protein Science |
Volume: | 7 |
Issue: | 10 |
Start Page: | 2150 |
End Page: | 2155 |
Abstract: | For echidna and canine milk lysozymes, which were presumed to be the calcium-binding lysozymes by their amino acid sequences, we have quantitated their calcium-binding strength and examined their guanidine unfolding profiles. The calcium-binding constants of echidna and canine lysozymes were determined to be 8.6 x 10(6) M(-1) and 8.9 x 10(6) M(-1) in 0.1 M KCl at pH 7.1 and 20 C, respectively. The unfolding of decalcified canine lysozyme proceeds in the same manner as that of alpha-lactalbumin, through a stable molten globule intermediate. However, neither calcium-bound nor decalcified echidna lysozyme shows a stable molten globule intermediate. This unfolding profile of echidna lysozyme is identical to that of conventional lysozymes and pigeon egg-white lysozyme, avian calcium-binding lysozyme. This result supports the suggestion of Prager and Jolles (Prager EM, Jolles P. 1996. Animal lysozymes c and g: An overview. In: Jolles P, ed. Lysozymes: Model enzymes in biochemistry and biology. Basel-Boston-Berlin: Birkhauzer Verlag. pp 9-31) that the lineage of avian and echidna calcium-binding lysozymes and that of eutherian calcium-binding lysozymes diverged separately from that of conventional lysozymes. |
Rights: | Copyright © 1998 Cambridge University Press |
Type: | article |
URI: | http://hdl.handle.net/2115/7394 |
Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 新田 勝利
|