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MicroRNA-101-5p Suppresses the Expression of the Ras-Related Protein RAP1A
| Title: | MicroRNA-101-5p Suppresses the Expression of the Ras-Related Protein RAP1A |
| Authors: | Shibayama, Yoshihiko Browse this author | | Kubo, Yoshitada Browse this author | | Nakagawa, Tsutomu Browse this author | | Iseki, Ken Browse this author →KAKEN DB |
| Keywords: | focal adhesion kinase (FAK) | | proliferation | | Rac | | RAS family protein | | salirasib |
| Issue Date: | Aug-2019 |
| Publisher: | The Pharmaceutical Society of Japan |
| Journal Title: | Biological & pharmaceutical bulletin |
| Volume: | 42 |
| Issue: | 8 |
| Start Page: | 1332 |
| End Page: | 1336 |
| Publisher DOI: | 10.1248/bpb.b19-00068 |
| Abstract: | MicroRNAs (miRs) are small, non-coding RNAs that negatively regulate gene expression. The stem loop sequence miR-101-1 generates mature miR-101-5p and miR-101-3p. The function and target mRNA of miR-101-5p have not yet been elucidated in detail. Here, we demonstrate that miR-101-5p inhibits the expression of RAP1A, a member of the RAS gene family. Transfection of a miR-101-5p mimic significantly inhibited the expression of RAP1A mRNA in HeLa, HEK293, A549, and COLO201 cells. The same treatment significantly inhibited cell proliferation. The cytostatic effect with transfection of miR-101-5p was antagonized by treatment with the RAP inhibitor salirasib. These results suggested that miR-101-5p inhibits RAP1A, and thus, the expression levels of miR-101-5p regulate cell proliferation. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/75351 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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