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MicroRNA-101-5p Suppresses the Expression of the Ras-Related Protein RAP1A

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Title: MicroRNA-101-5p Suppresses the Expression of the Ras-Related Protein RAP1A
Authors: Shibayama, Yoshihiko Browse this author
Kubo, Yoshitada Browse this author
Nakagawa, Tsutomu Browse this author
Iseki, Ken Browse this author →KAKEN DB
Keywords: focal adhesion kinase (FAK)
proliferation
Rac
RAS family protein
salirasib
Issue Date: Aug-2019
Publisher: The Pharmaceutical Society of Japan
Journal Title: Biological & pharmaceutical bulletin
Volume: 42
Issue: 8
Start Page: 1332
End Page: 1336
Publisher DOI: 10.1248/bpb.b19-00068
Abstract: MicroRNAs (miRs) are small, non-coding RNAs that negatively regulate gene expression. The stem loop sequence miR-101-1 generates mature miR-101-5p and miR-101-3p. The function and target mRNA of miR-101-5p have not yet been elucidated in detail. Here, we demonstrate that miR-101-5p inhibits the expression of RAP1A, a member of the RAS gene family. Transfection of a miR-101-5p mimic significantly inhibited the expression of RAP1A mRNA in HeLa, HEK293, A549, and COLO201 cells. The same treatment significantly inhibited cell proliferation. The cytostatic effect with transfection of miR-101-5p was antagonized by treatment with the RAP inhibitor salirasib. These results suggested that miR-101-5p inhibits RAP1A, and thus, the expression levels of miR-101-5p regulate cell proliferation.
Type: article
URI: http://hdl.handle.net/2115/75351
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

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