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安定同位体の天然存在量および安定同位体標識の検出法─2 : ガスクロマトグラフ-質量分析計とMassWorks
Title: | 安定同位体の天然存在量および安定同位体標識の検出法─2 : ガスクロマトグラフ-質量分析計とMassWorks |
Other Titles: | Stable isotope analysis -1: MassWorks on conventional gas chromatograph-mass spectrometer |
Authors: | 力石, 嘉人1 Browse this author →KAKEN DB | 滝沢, 侑子2 Browse this author →KAKEN DB | 布浦, 拓郎3 Browse this author |
Authors(alt): | Chikaraishi, Yoshito1 | Takiawa, Yuko2 | Nunoura, Takuro3 |
Keywords: | SIP 法 | 安定同位体 | アミノ酸 | 代謝 | SIP | Stable isotope | Amino acid | Metabolism |
Issue Date: | 20-Mar-2021 |
Publisher: | 低温科学第79巻編集委員会 |
Journal Title: | 低温科学 |
Journal Title(alt): | Low Temperature Science |
Volume: | 79 |
Start Page: | 23 |
End Page: | 29 |
Abstract: | 分子内Stable Isotope Probing 法とは,特定の部位を人工的に同位体標識した基質(ブドウ糖など)を用いて,その同位体標識の移動を,単一の生物体内,生物群集内,または海底の堆積場で追跡することで,試料中で起きている代謝合成経路の可視化や,生化学反応の特定を行う手法である.この手法は,近年,ガスクロクロマトグラフ-質量分析計(GC-MS)と質量のキャリブレーション用のソフトウェアMassWorks の組み合わせにより,大きく発展した.本稿では,この手法の原理について解説する. | MassWorks is an acquisition software that works on the mass spectra of conventional gas chromatograph-mass spectrometer, to determine stable isotope labels in organic compounds. The analysis of the isotope labels at the level of individual positions in organic compounds is potentially highly useful as a novel ‘Position-Specific Stable Isotope Probing’ to trace metabolic flow and associated function in diverse samples including single organisms and biotic community as well as sedimentary site. We review a brief outline of MassWorks and its application. |
Type: | bulletin (article) |
URI: | http://hdl.handle.net/2115/81347 |
Appears in Collections: | 低温科学 = Low Temperature Science > 第79巻
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