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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >
マイクロ流体デバイスを利用した下痢性貝毒オカダ酸の蛍光偏光免疫分析におけるブロッキング剤添加の影響
| Title: | マイクロ流体デバイスを利用した下痢性貝毒オカダ酸の蛍光偏光免疫分析におけるブロッキング剤添加の影響 |
| Other Titles: | Effects of Addition of Blocking Agents on Fluorescence Polarization Immunoassay of Okadaic Acid Using a Microfluidic Device |
| Authors: | 千田, 駿亮1 Browse this author | | 高橋, 和希2 Browse this author | | 福山, 真央3 Browse this author | | 粕谷, 素洋4 Browse this author | | 真栄城, 正寿5 Browse this author →KAKEN DB | | 石田, 晃彦6 Browse this author →KAKEN DB | | 谷, 博文7 Browse this author →KAKEN DB | | 重村, 幸治8 Browse this author | | ZHERDEV, Anatoly V.9 Browse this author | | EREMIN, Sergei A.10 Browse this author | | 火原, 彰秀11 Browse this author | | 渡慶次, 学12 Browse this author →KAKEN DB |
| Authors(alt): | CHIDA, Shunsuke1 | | TAKAHASHI, Kazuki2 | | FUKUYAMA, Mao3 | | KASUYA, Motohiro4 | | MAEKI, Masatoshi5 | | ISHIDA, Akihiko6 | | TANI, Hirofumi 7 | | SHIGEMURA, Koji 8 | | ZHERDEV, Anatoly V.9 | | EREMIN, Sergei A.10 | | HIBARA, Akihide11 | | TOKESHI, Manabu12 |
| Keywords: | microfluidic device | | polydimethylsiloxane | | fluorescence polarization immunoassay | | okadaic acid |
| Issue Date: | 5-Mar-2023 |
| Publisher: | 日本分析化学会 |
| Journal Title: | 分析化学 |
| Journal Title(alt): | BUNSEKI KAGAKU |
| Volume: | 72 |
| Issue: | 3 |
| Start Page: | 133 |
| End Page: | 138 |
| Publisher DOI: | 10.2116/bunsekikagaku.72.133 |
| Abstract: | マイクロ流体デバイスを利用した分析では，流路壁面への試料等の吸着が問題になる場合がある．特にポリジメチルシロキサン（PDMS）製デバイスを用いた免疫分析では，抗体や測定対象等の吸着が測定性能に大きく影響する．そこで本研究では，下痢性貝毒であるオカダ酸（OA）を測定対象として，蛍光偏光免疫分析によるオカダ酸の検出感度における二種類のブロックキング剤添加の影響について検討した．また，得られた検量線から抗体とトレーサーに対する解離定数を算出した． | | In analysis using microfluidic devices, adsorption of samples and other substances on the channel walls can be a problem. Especially in immunoassays using polydimethylsiloxane (PDMS) devices, the adsorption of antibodies and other substances to be measured greatly affects measurement performance. In this study, we investigated the effect of the addition of two blocking agents on the detection sensitivity of okadaic acid (OA), a diarrheal shellfish poison, by fluorescence polarization immunoassay. In addition, the dissociation constants for the antibody and tracer were calculated from the obtained calibration curve. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/89340 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 渡慶次 学
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