HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Global Institution for Collaborative Research and Education : GI-CoRE >
Peer-reviewed Journal Articles, etc >

First insight into the genetic population structure of Mycobacterium tuberculosis isolated from pulmonary tuberculosis patients in Egypt

This item is licensed under:Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International

Files in This Item:
MS_Diab-1st_TUBE_2015_252_Figs_2015.10.30_Submitted to Tuberculosis.docxFigures336.35 kBMicrosoft Word XMLView/Open
MS_Diab-1st_TUBE_2015_252_Main text_2015.10.30_Submitted to Tuberculosis-2.pdf735.32 kBPDFView/Open
MS_Diab-1st_TUBE_2015_252_Suppl File_2015.10.30_Submitted to Tuberculosis.docxSupplemental information251.85 kBMicrosoft Word XMLView/Open
MS_Diab-1st_TUBE_2015_252_Tables_Submitted to Tuberculosis.docxTables69.84 kBMicrosoft Word XMLView/Open
Please use this identifier to cite or link to this item:

Title: First insight into the genetic population structure of Mycobacterium tuberculosis isolated from pulmonary tuberculosis patients in Egypt
Authors: Diab, Hassan Mahmoud Browse this author
Nakajima, Chie Browse this author →KAKEN DB
Kotb, Saber A. Browse this author
Mokhtar, Alaa Browse this author
Khder, Nagwa F.M. Browse this author
Abdelaal, Ahmed S.A. Browse this author
Hegazy, Azza Browse this author
Poudel, Ajay Browse this author
Shah, Yogendra Browse this author
Suzuki, Yasuhiko Browse this author →KAKEN DB
Keywords: Genotyping
MTB lineages
Issue Date: Jan-2016
Publisher: Elsevier
Journal Title: Tuberculosis
Volume: 96
Start Page: 13
End Page: 20
Publisher DOI: 10.1016/
PMID: 26786649
Abstract: The present study aimed to assess the population structure of Mycobacterium tuberculosis (MTB) isolates from Egypt. A total of 230 MTB isolates were analysed using spoligotyping, large sequence polymorphism (LSPs), mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing and multi-locus sequence typing (MLST). The majority of isolates (93.0%) belonged to lineage 4, including 44.3, 13.4 and 10.8% of the ill-defined T clade, LAM and Haarlem families, respectively, and lineage 3 was identified in 7.0% of the isolates. MIRU-VNTRs typing allowed efficient discrimination of the spoligotype-defined clusters, including spoligo-international types (SIT) 53, 34, and 4, into 56 patterns, including 13 clusters and 43 unique patterns. A new SNP at position 311614 was identified in all six isolates to form the biggest MIRU-VNTR cluster, which suggested a recent clonal expansion. This SNP could possibly be used as a genetic marker for robust discriminations of Egyptian MTB isolates belonging to SIT53. The combination of spoligotyping, 12 MIRU-VNTRs loci and MLST provided insight into the genetic diversity and transmission dynamics of the Egyptian MTB genotypes and could be a key to implementation of effective control measures by public health authorities.
Rights: ©2015, Elsevier. This manuscript version is made available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Type: article (author version)
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鈴木 定彦

Export metadata:

OAI-PMH ( junii2 , jpcoar_1.0 )

MathJax is now OFF:


 - Hokkaido University