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Comprehensive lipidomic profiling in serum and multiple tissues from a mouse model of diabetes

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Title: Comprehensive lipidomic profiling in serum and multiple tissues from a mouse model of diabetes
Authors: Chen, Zhen Browse this author →KAKEN DB
Liang, Qiangrong Browse this author
Wu, Yue Browse this author
Gao, Zijun Browse this author
Kobayashi, Satoru Browse this author
Patel, Joy Browse this author
Li, Cairong Browse this author
Cai, Fei Browse this author
Zhang, Youhua Browse this author
Liang, Chongsheng Browse this author
Chiba, Hitoshi Browse this author →KAKEN DB
Hui, Shu-Ping Browse this author →KAKEN DB
Keywords: Lipidomics
Type 2 diabetes mellitus
Mouse model
Multiple tissues
Lipid hydroperoxides
Oxidative stress
Issue Date: 16-Oct-2020
Publisher: Springer
Journal Title: Metabolomics
Volume: 16
Issue: 11
Start Page: 115
Publisher DOI: 10.1007/s11306-020-01732-9
PMID: 33067714
Abstract: Introduction Diabetes mellitus is a serious metabolic disorder causing multiple organ damage in human. However, the lipidomic profiles in different organs and their associations are rarely studied in either diabetic patients or animals. Objectives To evaluate and compare the characteristics of lipid species in serum and multiple tissues in a diabetic mouse model. Methods Semi-quantitative profiling analyses of intact and oxidized lipids were performed in serum and multiple tissues from a diabetic mouse model fed a high fat diet and treated with streptozotocin by using LC/HRMS and MS/MS. The total content of each lipid class, and the tissue-specific lipid species in all tissue samples were determined and compared by multivariate analyses. Results The diabetic mouse model displayed characteristic differences in serum and multiple organs: the brain and heart showed the largest reduction in cardiolipin, while the kidney had more alterations in triacylglycerol. Interestingly, the lipidomic differences also existed between different regions of the same organ: cardiolipin species with highly polyunsaturated fatty acyls decreased only in atrium but not in ventricle, while renal cortex showed longer fatty acyl chains for both increased and decreased triacylglycerol species than renal medulla. Importantly, diabetes caused an accumulation of lipid hydroperoxides, suggesting that oxidative stress was induced in all organs except for the brain during the development of diabetes. Conclusions These findings provided novel insight into the organ-specific relationship between diabetes and lipid metabolism, which might be useful for evaluating not only diabetic tissue injury but also the effectiveness of diabetic treatments.
Rights: This is a post-peer-review, pre-copyedit version of an article published in Metabolomics. The final authenticated version is available online at:
Type: article (author version)
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 惠 淑萍

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