HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Global Institution for Collaborative Research and Education : GI-CoRE >
Peer-reviewed Journal Articles, etc >

Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand

Files in This Item:
MS_Utrarachkij_2nd_Accepted by MDR_Figure 1.ppt96.5 kBMicrosoft PowerpointView/Open
MS_Utrarachkij_2nd_Accepted by MDR_Figure 2.pptx7.64 MBMicrosoft Powerpoint XMLView/Open
MS_Utrarachkij_2nd_Accepted by MDR_Suppl Table 1.docx52.79 kBMicrosoft Word XMLView/Open
MS_Utrarachkij_2nd_Accepted by MDR_Suppl Table 2.docx40.49 kBMicrosoft Word XMLView/Open
MS_Utrarachkij_2nd_Accepted by MDR_Suppl Table 3.docx38.68 kBMicrosoft Word XMLView/Open
MS_Utrarachkij_2nd_Accepted by MDR_Table 1.docx37.78 kBMicrosoft Word XMLView/Open
MS_Utrarachkij_2nd_Accepted by MDR_Table 2.docx52.93 kBMicrosoft Word XMLView/Open
MS_Utrarachkij_2nd_Accepted by MDR_Table 3.docx59.5 kBMicrosoft Word XMLView/Open
MS_Utrarachkij_2nd_Accepted by MDR_Table 4.docx76.93 kBMicrosoft Word XMLView/Open
MS_Utrarachkij_2nd_Accepted by MDR_Table 5.docx39.62 kBMicrosoft Word XMLView/Open
Revised MS-3rd Rev_Utrarachkij_2nd_Main text_submitted to MDR_2016.11.13.pdf588.51 kBPDFView/Open
Revised MS_Utrarachkij_2nd_Table 6_Submitted to MDR_2016.02.29.docx16.63 kBMicrosoft Word XMLView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/71609

Title: Quinolone Resistance Determinants of Clinical Salmonella Enteritidis in Thailand
Authors: Utrarachkij, Fuangfa Browse this author
Nakajima, Chie Browse this author →KAKEN DB
Changkwanyeun, Ruchirada Browse this author
Siripanichgon, Kanokrat Browse this author
Kongsoi, Siriporn Browse this author
Pornruangwong, Srirat Browse this author
Changkaew, Kanjana Browse this author
Tsunoda, Risa Browse this author
Tamura, Yutaka Browse this author →KAKEN DB
Suthienkul, Orasa Browse this author
Suzuki, Yasuhiko Browse this author →KAKEN DB
Keywords: Salmonella Enteritidis
quinolone resistance
gyrA
qnrS1
MLVA
Issue Date: 1-Oct-2017
Publisher: Mary Ann Liebert
Journal Title: Microbial drug resistance
Volume: 23
Issue: 7
Start Page: 885
End Page: 894
Publisher DOI: 10.1089/mdr.2015.0234
PMID: 28437229
Abstract: Salmonella Enteritidis has emerged as a global concern regarding quinolone resistance and invasive potential. Although quinolone-resistant S. Enteritidis has been observed with high frequency in Thailand, information on the mechanism of resistance acquisition is limited. To elucidate the mechanism, a total of 158 clinical isolates of nalidixic acid (NAL)-resistant S. Enteritidis were collected throughout Thailand, and the quinolone resistance determinants were investigated in the context of resistance levels to NAL, norfloxacin (NOR), and ciprofloxacin (CIP). The analysis of point mutations in type II topoisomerase genes and the detection of plasmid-mediated quinolone resistance genes showed that all but two harbored a gyrA mutation, the qnrS1 gene, or both. The most commonly affected codon in mutant gyrA was 87, followed by 83. Double codon mutation in gyrA was found in an isolate with high-level resistance to NAL, NOR, and CIP. A new mutation causing serine to isoleucine substitution at codon 83 was identified in eight isolates. In addition to eighteen qnrS1-carrying isolates showing nontypical quinolone resistance, one carrying both the qnrS1 gene and a gyrA mutation also showed a high level of resistance. Genotyping by multilocus variable number of tandem repeat analysis suggested a possible clonal expansion of NAL-resistant strains nationwide. Our data suggested that NAL-resistant isolates with single quinolone resistance determinant may potentially become fluoroquinolone resistant by acquiring secondary determinants. Restricted therapeutic and farming usage of quinolones is strongly recommended to prevent the emergence of fluoroquinolone-resistant isolates.
Rights: Final publication is available from Mary Ann Liebert, Inc., publishers https://doi.org/10.1089/mdr.2015.0234
Type: article (author version)
URI: http://hdl.handle.net/2115/71609
Appears in Collections:国際連携研究教育局 : GI-CoRE (Global Institution for Collaborative Research and Education : GI-CoRE) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 鈴木 定彦

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University